Prof. Dr. FRANCESCO INCHINGOLO, MD, DMD, M.P.H
Born on September 12th, 1958 in Andria (Italy),
Medicine Doctor and specialized in:
1) Hygiene and Preventive Medicine (Master in Public Heath)
2) Dental Medicine
3) Maxillofacial Surgery
5) Experimental Medicine at the National Council of Research (C.N.R.).
Researcher and Assistant Professor in Dentistry at the School of Medicine, Department of
Interdisciplinary Medicine, University of Bari “Aldo MORO”, Italy. Professor of Oral and
Maxillofacial Surgery at the Maxillofacial Surgery Speciality Post Graduate course at the
University of Bari “Aldo Moro” Italy.
Principal Investigator in various Research Projects founded from University of Bari “Aldo Moro”.
Invited reviewer and Editorial Board Member in International Journals.
Head of the International Master Degree Course in Advanced Oral Surgery and Implant Surgery at
the University of Bari “Aldo Moro” Italy.
Invited Speaker in National and International courses and conferences.
Winner of “Sant’Apollonia Award” from ANDI (Italian Dental National Association) in year 1999
for the best clinical and scientific report in Dental Sciences.
Visiting Professor at the ” City Unity College- Greece – Cyprus” (and affiliated Institutions) and
BPP University London -UK.
Author of over 70 scientific papers in International indexed Journals with impact factor (h-index: 15
and Citations: 759 at 8th January 2018, Author ID: 6507557288), book chapters, 1 International
patent, and author of more than 200 articles in National Journals.
Pubmed link: http://www.ncbi.nlm.nih.gov/pubmed/?term=inchingolo+f
”NEW TECHNIQUES OF TISSUE REGERATIVE WITH GROWTH FACTOR AND STEM
CELLS: FROM INTRAORAL REGENERATION TO THE AESTHETIC MEDICINE ”
Prof. Dr. Francesco INCHINGOLO, PhD Gianna DIPALMA
University of Bari “Aldo Moro”, ITALY
Blood is considered the tissue which mostly encourages to the post-traumatic tissue regeneration,
thanks to its circulating components.
In our clinical reported cases the use of blood, together with the appropriate surgical techniques, has
been the most logical and performing procedure in regenerative therapy. It has allowed us to
encourage with easiness the healing process as the ONMG (OsteoNeoMorphoGenesis) posttraumatic or caused by atrophy and the treatment of aesthetic nature.
The C.G.F. (Concentrated Growth Factors) is a therapeutic protocol which is obtained by a
peripheral blood collection and separation of venous blood, at a constant temperature, through a
rotor (Medifughe) at an alternating and controlled speed.
In the end of the process of separation, the result consists in four characteristic steps in the tube:
A liquid phase represented by the serum (P.P.P.);
A superior phase of the tube represented by a block of polymerized fibrin in gel;
An intermediate phase containing Growth Factors, the cells of the white line, and stem cells;
An inferior phase, which is red and is represented by a dense clot which is rich in platelets.
The C.G.F, together with the CD 34+stem cells, has showed important results to our case report, in
terms of acceleration of the healing process, soft tissue repair process, and regeneration of the new
Through the degranulation of alpha granules of the platelets, C.G.F. is activated. In literature, these
are considered the most important growth factors which play an important role in the first step of
healing process of wounds, by accelerating it.
In this way the proliferation of cells, the formation of new matrix, the production of new osteoid
tissue are encouraged more and more, together with the connective tissue.
The C.G.F. takes action and increases the angiogenesis and the synthesis of the collagen, so that
healing time is accelerated. Growth Factors start their activity after very few minutes from the
beginning of the coagulation (indeed the dripping time can vary from 5 to 6 minutes, before that the
clot is created), for this reason the active secretion of these growth factors, starts within 5 or 6
minutes from the beginning of the lesion. This repairing phenomenon which interests more than
90% of growth factors is constant from the first hour until the following three days. Immediately
after this fast repairing process, there is a second step in which the different platelets secretes
further growth factors in the following 7 days of their life. The healing process of wound depends
on the subject, caused by the number of ongoing platelets (from 150.000 to 450.000) and the
clinical conditions of the patient (such as diabetes, blood diseases…).
For this reason there could be a different response according to the patient, his platelet
concentration in the blood clot in the graft or in the wound. The difference could concern time of
healing and the quality of tissue repair or neoformation.
In CGF there are also: cells TGF-b1, VEGF and CD34+.
In conclusion, as the C.G.F. is an organic matrix which is richer in fibrin and so it is denser than the
other platelet concentrates (for example P.R.P.), it could be used only in liquid form (A.P.A.G.
fillers), as membrane or together with other materials (deproteinized osteoinductive and/or
resorbable alloplastic materials as calcium triphosphate, autologous bone, and so on) in the way of
platelet gel englobing alloplastic materials which allow us to improve materials in granules which
are important for filling cavities and so increasing the bone volume. This technique is called Sticky
Bone (a new concept of fabricating growth factors-enriched bone graft matrix)
The use of C.G.F. for therapeutic purposes, represents a new, safe, standardized and cheaper
technology in many fields of medicine, orthopedics, ophthalmology, plastic surgery, maxilla facial,
oral surgery, ginaecology, aesthetic medicine, regenerative medicine, and so on.